Isabelle TARDIEUX |
||
Information |
||
Institute of Advanced Biosciences | ||
Biomechanics of Host-Apicomplexa Parasite Interactions | ||
Grenoble | ||
0000-0002-5677-7463 | ||
This email address is being protected from spambots. You need JavaScript enabled to view it. | ||
https://iab.univ-grenoble-alpes.fr/... | ||
@TardieuXlab | ||
Scientific interests and projects |
||
The team has a long lasting interest in combining molecular genetics and cell biology approaches to interrogate how forces and membrane dynamics co-operate during the obligate process of metazoan cell invasion by the Apicomplexa Toxoplasma gondii protozoan parasite. To this end, we have developed state-of-the art high-speed/high-resolution live imaging in conjunction with image processing and kinematic modeling of invading T. gondii tachyzoites. The team has more recently implemented in the lab force microscopy coupled with live fluorescent and super resolution microscopy and introduce nanoprobes/biosensors to also explore how forces can be generated by the free parasites to achieve exceptionally fast gliding in 2D & 3D micro-environments. Additional expertise including precision microfluidics and microfluidics-based micro-patterning, biomimetic and synthetic membrane systems, proteomics and structural biology has been recently achieved in the team, hence providing an ideal position at the interface between cell biology and biophysics to tackle down the yet unknown biomechanical properties of the Toxoplasma and their contribution to parasite fitness. In the context of ParaFrap, we plan continuing/starting to investigate in priority: 1- How the tachyzoite-derived forces coordinate with the host cell surface dynamic biomechanical properties during active invasion, 2- How the tachyzoite-derived forces cope with the parasite/parasite’ organelle deformability to ensure safe motion? 3- How physiologically relevant mechanical constraints could impact the nuclear compartment properties of T. gondii zoites with a focus on chromatin organization and gene expression? This particular research axis results from a tight collaboration with the the ParaFrap member (MH. Hakimi and team). |
||
Top 5 publications of the last 5 years |
||
1. Pavlou G, Milon G, Tardieux I. Intracellular protozoan parasites: living probes of the host cell surface molecular repertoire. Curr Opin Microbiol. 2019 Jul 23;52:116-123. Invited Review. PMID:31349210. doi: 10.1016/j.mib.2019.06.007. 2. Pavlou G, Biesaga M, Touquet B, Lagal V, Balland M, Dufour A, Hakimi MA, Tardieux I. Toxoplasma Parasite Twisting Motion Mechanically Induces Host Cell Membrane Fission to Complete Invasion within a Protective Vacuole. Cell Host Microbe. 2018 Jul 11;24(1):81-96.e5. Epub 2018 Jun 28. PMID: 30008293. doi: 10.1016/j.chom.2018.06.003 3. Tardieux I, Baum J. Reassessing the mechanics of parasite motility and host-cell invasion. J Cell Biol. 2016 Aug 29;214(5):507-15. Invited Review. PMID: 27573462. doi: 10.1083/jcb.201605100. 4. Bichet M, Touquet B, Gonzalez V, Florent I, Meissner M, Tardieux I. Genetic impairment of parasite myosin motors uncovers the contribution of host cell membrane dynamics to Toxoplasma invasion forces. BMC Biol. 2016 Nov 9;14(1):97. PMID: 27829452. doi: 10.1186/s12915-016-0316-8 5. Bichet M, Joly C, Henni AH, Guilbert T, Xémard M, Tafani V, Lagal V, Charras G, Tardieux I. The toxoplasma-host cell junction is anchored to the cell cortex to sustain parasite invasive force. BMC Biol. 2014 Dec 31;12:773. PMID: 25551479. doi: 10.1186/s12915-014-0108-y. |
The York Biomedical Research Institute (YBRI) are hosting an online live seminar on Friday 24th May 1-2pm BST as part of their biomedical science seminar series. You are all welcome to attend - registration is required via the...
[Communiqué] Congratulations to Arthur Talman, IRD Research Leader in the MIVEGEC laboratory (Montpellier), who has been awarded an Advanced Grant from the European Research Council (ERC) for the project "TROJAN - Molecular mimicry and immune...
Ana Rita Gomes, a CNRS Research Associate at the Laboratory of Pathogens and Host Immunity in Montpellier, and former Postdoctoral fellow of the LabEx ParaFrap, has been awarded the prestigious CNRS Bronze Medal in 2023. This...
. PhD Opportunity, Tours, France Supervision Thesis Supervisor: Sonia LAMANDE; Co-Supervisor: Julie TOTTEY Project: FUNTIONAL CHARACTERISATION OF CYSTEINE CATHEPSINS IN THE APICOMPLEXAN PARASITE CRYPTOSPORIDIUM...
Senior PostDoc position : computational biologist, Paris, France The ParSig team (https://research.pasteur.fr/en/team/molecular-parasitology-and-signaling) at the Institut Pasteur in Paris seeks for a highly...
Researcher in Livestock Immunology for the Study of Animal Trypanosomoses Through a One Health approach, the Intertryp Joint Research Unit (Cirad-IRD) is developing research on interactions between hosts,...
Happy New Year 2024! To kick off this new scientific year, here is the ParaFrap webinar schedule. Since January 2021, ParaFrap has been organizing a series of webinars. This monthly event, held every 2nd Thursday of the month, aims to strengthen...
PostDoc position : functional analysis of P. falciparum early gametocyte proteins, Allschwil, Switzerland The Malaria Gene Regulation Lab of Till Voss is looking for a highly motivated and talented postdoc to...
The team of Dr. Mohamed-Ali Hakimi, in collaboration with Isabelle Coppens (Johns Hopkins University Bloomberg School of Public Health and Malaria Research Institute, Baltimore, MD, USA), has developed a new (epi)genetic method to produce in vitro...
Success for ParaFrap students at MPM2023 Parafrap students won numerous awards at the MPM 2023, Woods Hole USA, 17-21 September 2023. A warm congratulations to all ! Parul Sharma (2nd year PhD under the...
© 2023. All rights reserved MLCOM
Notre site LabEx ParaFrap utilise des cookies pour réaliser des statistiques de visites, partager des contenus sur les réseaux sociaux et améliorer votre expérience. En refusant les cookies, certains services seront amenés à ne pas fonctionner correctement. Nous conservons votre choix pendant 30 jours. Vous pouvez changer d'avis en cliquant sur le bouton 'Cookies' en bas à gauche de chaque page de notre site. En savoir plus
Ce site utilise des cookies pour assurer son bon fonctionnement et ne peuvent pas être désactivés de nos systèmes. Nous ne les utilisons pas à des fins publicitaires. Si ces cookies sont bloqués, certaines parties du site ne pourront pas fonctionner.
Ce site utilise des cookies de mesure et d’analyse d’audience, tels que Google Analytics et Google Ads, afin d’évaluer et d’améliorer notre site internet.
Ce site utilise des composants tiers, tels que NotAllowedScript66291e1d99d2aReCAPTCHA, Google NotAllowedScript66291e1d998ffMaps, MailChimp ou Calameo, qui peuvent déposer des cookies sur votre machine. Si vous décider de bloquer un composant, le contenu ne s’affichera pas
Des plug-ins de réseaux sociaux et de vidéos, qui exploitent des cookies, sont présents sur ce site web. Ils permettent d’améliorer la convivialité et la promotion du site grâce à différentes interactions sociales.
Ce site web utilise un certain nombre de cookies pour gérer, par exemple, les sessions utilisateurs.