Marie-France |
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Information |
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TIMC UMR 5525 CNRS-Université Grenoble-Alpes | ||
BNI - Barrières Naturelles et Infectiosité | ||
Grenoble | ||
0000-0002-5423-4094 | ||
This email address is being protected from spambots. You need JavaScript enabled to view it. | ||
https://www-timc.imag.fr/en/bni | ||
Scientific interests and projects |
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The BNI team is interested in the innate control of Toxoplasma gondii infectivity. Infectivity refers to the capacity of a parasite to infect an individual. It involves pathogenrecognition systems, and the diversification of responses relies on the combined genetic diversity of both host and pathogen. We have established that in the rat model the control of Toxoplasma infectivity is genetically controlled by Toxo1, a single locus located on the rat chromosome 10. In resistant rats, the parasite elimination occurs at the site of infection (intestinal barrier) and involves the rapid killing of infected resident macrophages which in turn contributes to the lack of parasite dissemination. In vitro, infected resistant rat macrophages trigger the NOD-like receptor NLRP1a/Caspase-1 pathway to induce the death of both parasites and their hosting macrophages. This is parasite-induced and differs among T. gondii genotypes. Thus, having demonstrated that the parasite fate depends on both the Toxo1 locus genotype (LEW or BN origin) and the parasite genotype, we are interested in the characterization of factor(s) and pathway(s) which activate the Toxo1- dependent resistance to toxoplasmosis. Our ongoing strategy is based on global comparative analyses of -i) resistant vs susceptible macrophages functions before and following parasite infection and -ii) a set of parasite mutant vs wildtype capable to bypass the resistance. Bioinformatics analysis will combine results from comparative proteomics, transcriptomics and metabolics. Candidate signaling/metabolic host pathway(s) and gene(s) will be validated using classical reverse genetics, biochemical and cellular studies |
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Top 5 publications of the last 5 years |
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1. Cavailles P, Flori P, Papapietro O, Bisanz C, Massera C, Cristinelli S, Jublot D, Bastien O, Loeuillet C, Aldebert D, Touquet Lagrange D, Pilloux Ludovic, B, Fournié GJ and MF Cesbron-Delauw (2014) A highly conserved Toxo1 haplotype directs resistance to toxoplasmosis and its associated caspase-1 dependent killing of parasite and host macrophage. PLoS Path. 10(4), e1004005. doi: 10.1371/journal.ppat.1004005 2. Mercier C and MF Cesbron-Delauw (2015) Toxoplasma secretory granules: one population or more? Trends Parasitol. 31: 60-71. doi: 10.1016/j.pt.2014.12.002 3. Lopez J., Bittame A, Massera C, Vasseur V, Effantin G, Valat A, Buaillon C, Allart S, Fox BA, Rommereim LM, Bzik DJ, Schoen G, Weissenhorn W, Dubremetz JF, Gagnon J, Mercier C, Cesbron-Delauw MF and N Blanchard (2015) Intravacuolar membranes regulate CD8 T cell recognition of membrane-bound Toxoplasma gondii protective antigen. Cell Reports 13: 2273-2286. doi: 10.1016/j.celrep.2015.11.001 4. Touquet B, Pellissier E, Cavailles P, Yi W, Bellini V, Mercier M, Cesbron-Delauw MF, Boumendjel A and D Aldebert (2018) High-content imaging assay to evaluate Toxoplasma gondii infection and proliferation: a multiparametric assay to screen new compounds. PLoS One 13(8):e0201678. doi: https://doi.org/10.1371/journal.pone.0201678. 5. Loeuillet C., Mondon A., Kamche S., Curri V., Boutonnat J., Cavaillès P. and MF Cesbron- Delauw (2019). Toxoplasma hypervirulence in the rat model parallels human infection and is modulated by the Toxo1 locus. Front. Cell. Infection Microbiol. 9 :13 doi: 10.3389/fcimb.2019.00134 |
The York Biomedical Research Institute (YBRI) are hosting an online live seminar on Friday 24th May 1-2pm BST as part of their biomedical science seminar series. You are all welcome to attend - registration is required via the...
[Communiqué] Congratulations to Arthur Talman, IRD Research Leader in the MIVEGEC laboratory (Montpellier), who has been awarded an Advanced Grant from the European Research Council (ERC) for the project "TROJAN - Molecular mimicry and immune...
Ana Rita Gomes, a CNRS Research Associate at the Laboratory of Pathogens and Host Immunity in Montpellier, and former Postdoctoral fellow of the LabEx ParaFrap, has been awarded the prestigious CNRS Bronze Medal in 2023. This...
. PhD Opportunity, Tours, France Supervision Thesis Supervisor: Sonia LAMANDE; Co-Supervisor: Julie TOTTEY Project: FUNTIONAL CHARACTERISATION OF CYSTEINE CATHEPSINS IN THE APICOMPLEXAN PARASITE CRYPTOSPORIDIUM...
Senior PostDoc position : computational biologist, Paris, France The ParSig team (https://research.pasteur.fr/en/team/molecular-parasitology-and-signaling) at the Institut Pasteur in Paris seeks for a highly...
Researcher in Livestock Immunology for the Study of Animal Trypanosomoses Through a One Health approach, the Intertryp Joint Research Unit (Cirad-IRD) is developing research on interactions between hosts,...
Happy New Year 2024! To kick off this new scientific year, here is the ParaFrap webinar schedule. Since January 2021, ParaFrap has been organizing a series of webinars. This monthly event, held every 2nd Thursday of the month, aims to strengthen...
PostDoc position : functional analysis of P. falciparum early gametocyte proteins, Allschwil, Switzerland The Malaria Gene Regulation Lab of Till Voss is looking for a highly motivated and talented postdoc to...
The team of Dr. Mohamed-Ali Hakimi, in collaboration with Isabelle Coppens (Johns Hopkins University Bloomberg School of Public Health and Malaria Research Institute, Baltimore, MD, USA), has developed a new (epi)genetic method to produce in vitro...
Success for ParaFrap students at MPM2023 Parafrap students won numerous awards at the MPM 2023, Woods Hole USA, 17-21 September 2023. A warm congratulations to all ! Parul Sharma (2nd year PhD under the...
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